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The medium additives such as fatty acid, lipid, mannose, folic acid, CaCl₂ were examined to enhance recombinant β-galactosidase (β-gal) production in T-flask and air-lift bioreactor. The addition of each component. such as cholesterol, tocopherol, tricaprylin, mannose and folic acid at AcNPV infection of Tn5B1-4 cells enhanced β-gal production, whereas the addition of CaCl₂ did not increase β-gal production. The recombinant β-gal production using the infection medium supplemented with a mixture of 0.34 mM cholesterol, 2.2 mM mannose and 0.045 M folic acid was enhanced 2 fold in an air-lift bioreactor, compared to the basal medium.
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